Functionalized scaffold-mediated interleukin 10 gene delivery significantly improves survival rates of stem cells in vivo.

ABSTRACT: While stem cell transplantation could potentially treat a variety of disorders, clinical studies have not yet demonstrated conclusive benefits. This may be partly because transplanted stem cells have low survival rates, potentially due to host inflammation. The system described herein used two different gene therapy techniques to improve retention of rat mesenchymal stem cells. In the first, stem cells were transfected with interleukin-10 (IL-10) before being loaded into a collagen scaffold. In the second, unmodified stem cells were loaded into a collagen scaffold along with polymer-complexed IL-10 plasmids. The scaffolds were surgically implanted into the dorsum of syngeneic rats. At each endpoint, the scaffolds were explanted and cell retention, IL-10 level and inflammatory response were

SUBMITTER: Holladay C 

PROVIDER: S-EPMC3086863 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature