Proteomic analyses reveal common promiscuous patterns of cell surface proteins on human embryonic stem cells and sperms.

ABSTRACT:

Background

It has long been proposed that early embryos and reproductive organs exhibit similar gene expression profiles. However, whether this similarity is propagated to the protein level remains largely unknown. We have previously characterised the promiscuous expression pattern of cell surface proteins on mouse embryonic stem (mES) cells. As cell surface proteins also play critical functions in human embryonic stem (hES) cells and germ cells, it is important to reveal whether a promiscuous pattern of cell surface proteins also exists for these cells.

Methods and principal findings

Surface proteins of hES cells and human mature sperms (hSperms) were purified by biotin labelling and subjected to proteomic analyses. More than 1000 transmembrane or secreted cell surface prot

SUBMITTER: Gu B 

PROVIDER: S-EPMC3086920 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature