Global gene expression profiling and validation in esophageal squamous cell carcinoma and its association with clinical phenotypes.

ABSTRACT:

Purpose

Esophageal squamous cell carcinoma (ESCC) is an aggressive tumor with poor prognosis. Understanding molecular changes in ESCC will enable identification of molecular subtypes and provide potential targets for early detection and therapy.

Experimental design

We followed up a previous array study with additional discovery and confirmatory studies in new ESCC cases by using alternative methods. We profiled global gene expression for discovery and confirmation, and validated selected dysregulated genes with additional RNA and protein studies.

Results

A total of 159 genes showed differences with extreme statistical significance (P < E-15) and 2-fold differences or more in magnitude (tumor/normal RNA expression ratio, N = 53 cases), including 116 upregulated and 43

SUBMITTER: Su H 

PROVIDER: S-EPMC3086948 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature