Role and relevance of TrkB mutations and expression in non-small cell lung cancer.

ABSTRACT:

Purpose

TrkB has been involved in poor cancer outcome. TrkB mutations have been reported in non-small cell lung cancer. In this study, we aimed at characterizing the role of three potentially sensitizing TrkB mutations previously reported in lung cancer.

Experimental design

We characterized three activation loop mutants of TrkB (M713I, R715G, and R734C) in terms of pathway activation/phosphorylation, migration, anchorage-independent growth, and sensitivity to a Trk inhibitor, using NIH3T3 cells and Baf3 cells. We also sequenced the tyrosine kinase domain of TrkB in a large number of lung cancer samples of East-Asian origin and cell lines.

Results

None of the mutants were constitutively active in NIH3T3 transformation and migration assays. M713I and R734C mutants showe

SUBMITTER: Harada T 

PROVIDER: S-EPMC3086954 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature