Inhibitor of PI3Kγ ameliorates TNBS-induced colitis in mice by affecting the functional activity of CD4+CD25+FoxP3+ regulatory T cells.

ABSTRACT:

Background and purpose

Phosphoinositide 3-kinase-γ (PI3Kγ) is implicated in many pathophysiological conditions, and recent evidence has suggested its involvement in colitis. In the present study, we investigated the effects of AS605240, a relatively selective PI3Kγ inhibitor, in experimental colitis and its underlying mechanisms.

Experimental approach

Acute colitis was induced in mice by treatment with trinitrobenzene sulphonic acid (TNBS), and the effect of AS605240 on colonic injury was assessed. Pro-inflammatory mediators and cytokines were measured by immunohistochemistry, elisa, real time-polymerase chain reaction and flow cytometry.

Key results

Oral administration of AS605240 significantly attenuated TNBS-induced acute colitis and diminished the expression of ma

SUBMITTER: Dutra RC 

PROVIDER: S-EPMC3087137 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature