The Sry3 Y chromosome locus elevates blood pressure and renin-angiotensin system indexes.

ABSTRACT:

Background

Sex-determining region Y (Sry) is a transcription factor. Our research group has shown that there are multiple copies of Sry in Wistar-Kyoto (WKY) and spontaneous hypertensive (SHR) rats, and that they have novel functions separate from testes determination.

Objective

We hypothesized that exogenously delivered Sry3 to the normotensive WKY male kidney would activate the renin-angiotensin system (RAS) and raise blood pressure (BP), based on previous in vitro studies.

Methods

Sry3 or control vector was electroporated to the left kidney of male WKY rats and the following measurements were taken: BP by telemetry, renin-angiotensin measures by radioimmunoassay, plasma and tissue catecholamines by HPLC with electrochemical detection, sodium by flame photometry, an

SUBMITTER: Ely D 

PROVIDER: S-EPMC3087190 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature