Identification of expressed genes during compatible interaction between stripe rust (Puccinia striiformis) and wheat using a cDNA library.

ABSTRACT:

Background

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat worldwide. To establish compatibility with the host, Pst forms special infection structures to invade the plant with minimal damage to host cells. Although compatible interaction between wheat and Pst has been studied using various approaches, research on molecular mechanisms of the interaction is limited. The aim of this study was to develop an EST database of wheat infected by Pst in order to determine transcription profiles of genes involved in compatible wheat-Pst interaction.

Results

Total RNA, extracted from susceptible infected wheat leaves harvested at 3, 5 and 8 days post inoculation (dpi), was used to create a cDNA library, from which

SUBMITTER: Ma J 

PROVIDER: S-EPMC3087560 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature