A robust, simple genotyping-by-sequencing (GBS) approach for high diversity species.

ABSTRACT: Advances in next generation technologies have driven the costs of DNA sequencing down to the point that genotyping-by-sequencing (GBS) is now feasible for high diversity, large genome species. Here, we report a procedure for constructing GBS libraries based on reducing genome complexity with restriction enzymes (REs). This approach is simple, quick, extremely specific, highly reproducible, and may reach important regions of the genome that are inaccessible to sequence capture approaches. By using methylation-sensitive REs, repetitive regions of genomes can be avoided and lower copy regions targeted with two to three fold higher efficiency. This tremendously simplifies computationally challenging alignment problems in species with high levels of genetic diversity. The GBS procedure is demon

SUBMITTER: Elshire RJ 

PROVIDER: S-EPMC3087801 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature