Olfactomedin 2: expression in the eye and interaction with other olfactomedin domain-containing proteins.

ABSTRACT:

Purpose

Olfactomedin 2 (OLFM2) belongs to the family of olfactomedin domain-containing proteins. Genetic data suggest its association with glaucoma in Japanese patients. However, its functions are still elusive. In this study, the properties of mammalian OLFM2 were investigated.

Methods

Expression of the rat and mouse Olfm2 gene was studied by using real-time PCR and in situ hybridization. Substitutions were introduced into OLFM2 by mutagenesis in vitro. Intracellular localization of OLFM2 was studied by confocal microscopy after transient transfection in HEK293 cells. Interaction of OLFM2 with olfactomedin 1 (Olfm1), olfactomedin 3 (Olfm3), myocilin, and gliomedin was studied by using co-immunoprecipitation.

Results

Two major human OLFM2 mRNAs encode secreted protein

SUBMITTER: Sultana A 

PROVIDER: S-EPMC3088551 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature