Intravitreal injection of AAV2 transduces macaque inner retina.

ABSTRACT:

Purpose

Adeno-associated virus serotype 2 (AAV2) has been shown to be effective in transducing inner retinal neurons after intravitreal injection in several species. However, results in nonprimates may not be predictive of transduction in the human inner retina, because of differences in eye size and the specialized morphology of the high-acuity human fovea. This was a study of inner retina transduction in the macaque, a primate with ocular characteristics most similar to that of humans.

Methods

In vivo imaging and histology were used to examine GFP expression in the macaque inner retina after intravitreal injection of AAV vectors containing five distinct promoters.

Results

AAV2 produced pronounced GFP expression in inner retinal cells of the fovea, no expression in t

SUBMITTER: Yin L 

PROVIDER: S-EPMC3088562 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature