Cross-sectional characterization of HIV-1 env compartmentalization in cerebrospinal fluid over the full disease course.

ABSTRACT:

Objectives

To characterize HIV-1 env compartmentalization between cerebrospinal fluid (CSF) and peripheral blood plasma over all stages of the HIV-1 disease course, and to determine the relationship between the extent of CSF HIV-1 env compartmentalization and clinical neurologic disease status.

Design

Paired blood plasma and CSF specimens were collected from 66 HIV-infected patients cross-sectionally representing all major clinical stages relating to HIV-associated neurologic disease, including primary infection, asymptomatic chronic infection, chronic infection with minor global impairment, and immune deficiency with HIV-associated dementia.

Methods

Heteroduplex tracking assays and bulk sequence analysis targeting the V1/V2, C2-V3, and V4/V5 regions of env were perfo

SUBMITTER: Harrington PR 

PROVIDER: S-EPMC3089803 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature