Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system.

ABSTRACT:

Background

Recently, some groups have reported on cell-free synthesis of functional membrane proteins (MPs) in the presence of exogenous liposomes (liposomes). Previously, we reported synthesis of a functional AtPPT1 plant phosphate transporter that was associated with liposomes during translation. However, it is unclear whether or not lipid/MP complex formation is common to all types of MPs in the wheat cell-free system.

Results

AtPPT1 was synthesized using a wheat cell-free system with or without liposomes. AtPPT1 synthesized with liposomes showed high transport activity, but the activity of AtPPT1 synthesized without liposomes was less than 10% activity of that with liposomes. To test whether co-translational association with liposomes is observed in the synthesis of othe

SUBMITTER: Nozawa A 

PROVIDER: S-EPMC3090341 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature