Dissecting the specificity of protein-protein interaction in bacterial two-component signaling: orphans and crosstalks.

ABSTRACT: Predictive understanding of the myriads of signal transduction pathways in a cell is an outstanding challenge of systems biology. Such pathways are primarily mediated by specific but transient protein-protein interactions, which are difficult to study experimentally. In this study, we dissect the specificity of protein-protein interactions governing two-component signaling (TCS) systems ubiquitously used in bacteria. Exploiting the large number of sequenced bacterial genomes and an operon structure which packages many pairs of interacting TCS proteins together, we developed a computational approach to extract a molecular interaction code capturing the preferences of a small but critical number of directly interacting residue pairs. This code is found to reflect physical interaction mechani

SUBMITTER: Procaccini A 

PROVIDER: S-EPMC3090404 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature