Truncating the i-leader open reading frame enhances release of human adenovirus type 5 in glioma cells.

ABSTRACT:

Background

The survival of glioma patients with the current treatments is poor. Early clinical trails with replicating adenoviruses demonstrated the feasibility and safety of the use of adenoviruses as oncolytic agents. Antitumor efficacy has been moderate due to inefficient virus replication and spread. Previous studies have shown that truncation of the adenovirus i-leader open reading frame enhanced cytopathic activity of HAdV-5 in several tumor cell lines. Here we report the effect of an i-leader mutation on the cytopathic activity in glioma cell lines and in primary high-grade glioma cell cultures.

Results

A mutation truncating the i-leader open reading frame was created in a molecular clone of replication-competent wild-type HAdV-5 by site-directed mutagenesis. We analy

SUBMITTER: van den Hengel SK 

PROVIDER: S-EPMC3090740 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature