Structurally distinct LewisX glycans distinguish subpopulations of neural stem/progenitor cells.

ABSTRACT: There is increasing evidence that the stem and progenitor cell population that builds the central nervous system is very heterogeneous. Stem cell markers with the potential to divide this cell pool into subpopulations with distinct characteristics are sparse. We were looking for new cell type-specific antigens to further subdivide the progenitor pool. Here, we introduce the novel monoclonal antibody clone 5750. We show that it specifically labels cell surfaces of neural stem and progenitor cells. When 5750-expressing cells were isolated by fluorescence-activated cell sorting from embryonic mouse brains, the sorted population showed increased neurosphere forming capacity and multipotency. Neurospheres generated from 5750-positive cells could self-renew and remained multipotent even after pr

SUBMITTER: Hennen E 

PROVIDER: S-EPMC3091238 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature