Ligand-dependent dynamics of retinoic acid receptor binding during early neurogenesis.

ABSTRACT:

Background

Among its many roles in development, retinoic acid determines the anterior-posterior identity of differentiating motor neurons by activating retinoic acid receptor (RAR)-mediated transcription. RAR is thought to bind the genome constitutively, and only induce transcription in the presence of the retinoid ligand. However, little is known about where RAR binds to the genome or how it selects target sites.

Results

We tested the constitutive RAR binding model using the retinoic acid-driven differentiation of mouse embryonic stem cells into differentiated motor neurons. We find that retinoic acid treatment results in widespread changes in RAR genomic binding, including novel binding to genes directly responsible for anterior-posterior specification, as well as the subs

SUBMITTER: Mahony S 

PROVIDER: S-EPMC3091300 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature