Long-term live imaging provides new insight into stem cell regulation and germline-soma coordination in the Drosophila ovary.

ABSTRACT: The Drosophila ovariole tip produces new ovarian follicles on a 12-hour cycle by controlling niche-based germline and follicle stem cell divisions and nurturing their developing daughters. Static images provide a thumbnail view of folliculogenesis but imperfectly capture the dynamic cellular interactions that underlie follicle production. We describe a live-imaging culture system that supports normal ovarian stem cell activity, cyst movement and intercellular interaction over 14 hours, which is long enough to visualize all the steps of follicle generation. Our results show that live imaging has unique potential to address diverse aspects of stem cell biology and gametogenesis. Stem cells in cultured tissue respond to insulin and orient their mitotic spindles. Somatic escort cells, the glia

SUBMITTER: Morris LX 

PROVIDER: S-EPMC3091492 | biostudies-literature | 2011 Jun

REPOSITORIES: biostudies-literature