Development and diversity of Andean-derived, gene-based microsatellites for common bean (Phaseolus vulgaris L.).

ABSTRACT:

Background

Gene-based (genic) microsatellites are a useful tool for plant genetics and simple sequence repeat loci can often be found in coding regions of the genome. While EST sequencing can be used to discover genic microsatellites, direct screening of cDNA libraries for repeat motifs can save on overall sequencing costs. The objective of this research was to screen a large cDNA library from and Andean common bean genotype for six di-nucleotide and tri-nucleotide repeat motifs through a filter hybridization approach and to develop microsatellite markers from positive clones.

Results

Robotics were used for high-throughput colony picking and to create a high-density filter of 18,432 double spotted cDNA clones which was followed by hybridization with repeat motif containing p

SUBMITTER: Blair MW 

PROVIDER: S-EPMC3091531 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature