Array comparative genomic hybridization analysis of Trichoderma reesei strains with enhanced cellulase production properties.

ABSTRACT:

Background

Trichoderma reesei is the main industrial producer of cellulases and hemicellulases that are used to depolymerize biomass in a variety of biotechnical applications. Many of the production strains currently in use have been generated by classical mutagenesis. In this study we characterized genomic alterations in high-producing mutants of T. reesei by high-resolution array comparative genomic hybridization (aCGH). Our aim was to obtain genome-wide information which could be utilized for better understanding of the mechanisms underlying efficient cellulase production, and would enable targeted genetic engineering for improved production of proteins in general.

Results

We carried out an aCGH analysis of four high-producing strains (QM9123, QM9414, NG14 and Rut-C30) us

SUBMITTER: Vitikainen M 

PROVIDER: S-EPMC3091638 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature