Transcriptional profiling of host gene expression in chicken embryo lung cells infected with laryngotracheitis virus.

ABSTRACT:

Background

Infection by infectious laryngotracheitis virus (ILTV; gallid herpesvirus 1) causes acute respiratory diseases in chickens often with high mortality. To better understand host-ILTV interactions at the host transcriptional level, a microarray analysis was performed using 4 x 44 K Agilent chicken custom oligo microarrays.

Results

Microarrays were hybridized using the two color hybridization method with total RNA extracted from ILTV infected chicken embryo lung cells at 0, 1, 3, 5, and 7 days post infection (dpi). Results showed that 789 genes were differentially expressed in response to ILTV infection that include genes involved in the immune system (cytokines, chemokines, MHC, and NF-kappaB), cell cycle regulation (cyclin B2, CDK1, and CKI3), matrix metalloproteina

SUBMITTER: Lee JY 

PROVIDER: S-EPMC3091642 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature