An insight into the sialotranscriptome of the brown dog tick, Rhipicephalus sanguineus.

ABSTRACT:

Background

Rhipicephalus sanguineus, known as the brown dog tick, is a common ectoparasite of domestic dogs and can be found worldwide. R.sanguineus is recognized as the primary vector of the etiological agent of canine monocytic ehrlichiosis and canine babesiosis. Here we present the first description of a R. sanguineus salivary gland transcriptome by the production and analysis of 2,034 expressed sequence tags (EST) from two cDNA libraries, one consctructed using mRNA from dissected salivary glands from female ticks fed for 3-5 days (early to mid library, RsSGL1) and the another from ticks fed for 5 days (mid library, RsSGL2), identifying 1,024 clusters of related sequences.

Results

Based on sequence similarities to nine different databases, we identified transcripts of ge

SUBMITTER: Anatriello E 

PROVIDER: S-EPMC3091647 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature