A proteogenomic update to Yersinia: enhancing genome annotation.

ABSTRACT:

Background

Modern biomedical research depends on a complete and accurate proteome. With the widespread adoption of new sequencing technologies, genome sequences are generated at a near exponential rate, diminishing the time and effort that can be invested in genome annotation. The resulting gene set contains numerous errors in even the most basic form of annotation: the primary structure of the proteins.

Results

The application of experimental proteomics data to genome annotation, called proteogenomics, can quickly and efficiently discover misannotations, yielding a more accurate and complete genome annotation. We present a comprehensive proteogenomic analysis of the plague bacterium, Yersinia pestis KIM. We discover non-annotated genes, correct protein boundaries, remove sp

SUBMITTER: Payne SH 

PROVIDER: S-EPMC3091656 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature