The Escherichia coli K-12 ORFeome: a resource for comparative molecular microbiology.

ABSTRACT:

Background

Systems biology and functional genomics require genome-wide datasets and resources. Complete sets of cloned open reading frames (ORFs) have been made for about a dozen bacterial species and allow researchers to express and study complete proteomes in a high-throughput fashion.

Results

We have constructed an open reading frame (ORFeome) collection of 3974 or 94% of the known Escherichia coli K-12 ORFs in Gateway entry vector pENTR/Zeo. The collection has been used for protein expression and protein interaction studies. For example, we have compared interactions among YgjD, YjeE and YeaZ proteins in E. coli, Streptococcus pneumoniae, and Staphylococcus aureus. We also compare this ORFeome with other Gateway-compatible bacterial ORFeomes and show its utility for comp

SUBMITTER: Rajagopala SV 

PROVIDER: S-EPMC3091666 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature