Genetic validation of whole-transcriptome sequencing for mapping expression affected by cis-regulatory variation.

ABSTRACT:

Background

Identifying associations between genotypes and gene expression levels using microarrays has enabled systematic interrogation of regulatory variation underlying complex phenotypes. This approach has vast potential for functional characterization of disease states, but its prohibitive cost, given hundreds to thousands of individual samples from populations have to be genotyped and expression profiled, has limited its widespread application.

Results

Here we demonstrate that genomic regions with allele-specific expression (ASE) detected by sequencing cDNA are highly enriched for cis-acting expression quantitative trait loci (cis-eQTL) identified by profiling of 500 animals in parallel, with up to 90% agreement on the allele that is preferentially expressed. We also ob

SUBMITTER: Babak T 

PROVIDER: S-EPMC3091669 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature