Cell-specific occupancy of an extended repertoire of CREM and CREB binding loci in male germ cells.

ABSTRACT:

Background

CREB and CREM are closely related factors that regulate transcription in response to various stress, metabolic and developmental signals. The CREMτ activator isoform is selectively expressed in haploid spermatids and plays an essential role in murine spermiogenesis.

Results

We have used chromatin immunoprecipitation coupled to sequencing (ChIP-seq) to map CREM and CREB target loci in round spermatids from adult mouse testis and spermatogonia derived GC1-spg cells respectively. We identify more than 9000 genomic loci most of which are cell-specifically occupied. Despite the fact that round spermatids correspond to a highly specialised differentiated state, our results show that they have a remarkably accessible chromatin environment as CREM occupies more than 6700

SUBMITTER: Martianov I 

PROVIDER: S-EPMC3091680 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature