Characterization of the rainbow trout transcriptome using Sanger and 454-pyrosequencing approaches.

ABSTRACT:

Background

Rainbow trout are important fish for aquaculture and recreational fisheries and serves as a model species for research investigations associated with carcinogenesis, comparative immunology, toxicology and evolutionary biology. However, to date there is no genome reference sequence to facilitate the development of molecular technologies that utilize high-throughput characterizations of gene expression and genetic variation. Alternatively, transcriptome sequencing is a rapid and efficient means for gene discovery and genetic marker development. Although a large number (258,973) of EST sequences are publicly available, the nature of rainbow trout duplicated genome hinders assembly and complicates annotation.

Results

High-throughput deep sequencing of the Swanson rain

SUBMITTER: Salem M 

PROVIDER: S-EPMC3091713 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature