Deciphering the intracellular metabolism of Listeria monocytogenes by mutant screening and modelling.

ABSTRACT:

Background

The human pathogen Listeria monocytogenes resides and proliferates within the cytoplasm of epithelial cells. While the virulence factors essentially contributing to this step of the infection cycle are well characterized, the set of listerial genes contributing to intracellular replication remains to be defined on a genome-wide level.

Results

A comprehensive library of L. monocytogenes strain EGD knockout mutants was constructed upon insertion-duplication mutagenesis, and 1491 mutants were tested for their phenotypes in rich medium and in a Caco-2 cell culture assay. Following sequencing of the plasmid insertion site, 141 different genes required for invasion of and replication in Caco-2 cells were identified. Ten in-frame deletion mutants were constructed that co

SUBMITTER: Schauer K 

PROVIDER: S-EPMC3091722 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature