EcoTILLING in Capsicum species: searching for new virus resistances.

ABSTRACT:

Background

The EcoTILLING technique allows polymorphisms in target genes of natural populations to be quickly analysed or identified and facilitates the screening of genebank collections for desired traits. We have developed an EcoTILLING platform to exploit Capsicum genetic resources. A perfect example of the utility of this EcoTILLING platform is its application in searching for new virus-resistant alleles in Capsicum genus. Mutations in translation initiation factors (eIF4E, eIF(iso)4E, eIF4G and eIF(iso)4G) break the cycle of several RNA viruses without affecting the plant life cycle, which makes these genes potential targets to screen for resistant germplasm.

Results

We developed and assayed a cDNA-based EcoTILLING platform with 233 cultivated accessions of the genus Ca

SUBMITTER: Ibiza VP 

PROVIDER: S-EPMC3091766 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature