Spatial and temporal profiles of cytokinin biosynthesis and accumulation in developing caryopses of maize.

ABSTRACT:

Background and aims

Cytokinins are a major group of plant hormones and are associated with various developmental processes. Developing caryopses of maize have high levels of cytokinins, but little is known about their spatial and temporal distribution. The localization and quantification of cytokinins was investigated in maize (Zea mays) caryopsis from 0 to 28 d after pollination together with the expression and localization of isopentenyltransferase ZmIPT1 involved in cytokinin biosynthesis and ZmCNGT, the gene putatively involved in N9-glucosylation.

Methods

Biochemical, cellular and molecular approaches resolved the overall cytokinin profiles, and several gene expression assays were used for two critical genes to assess cytokinin cell-specific biosynthesis and conversion

SUBMITTER: Rijavec T 

PROVIDER: S-EPMC3091798 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature