Glycogen synthase kinase-3 negatively regulates tissue factor expression in monocytes interacting with activated platelets.

ABSTRACT:

Background

At the site of vascular injury, monocytes (MN) interacting with activated platelets (PLT) synthesize tissue factor (TF) and promote thrombus formation. Intracellular signals necessary for the expression of TF in MN, in the context of a developing thrombus, remain unknown.

Objective

The study was designed to investigate the role of the glycogen synthase kinase 3 (GSK3, a serine-threonine kinase) downstream insulin receptor pathway, in PLT-induced TF expression in MN.

Methods

To this purpose we used a well-characterized in vitro model of human MN-PLT interactions that allows detailed analysis of TF activity, TF protein and gene expression.

Results

The results demonstrated that, in MN interacting with activated PLT: (i) TF activity, antigen and mRNA wer

SUBMITTER: Di Santo A 

PROVIDER: S-EPMC3091995 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature