Binding and "pKa" modulation of a polycyclic substrate analogue in a type II polyketide acyl carrier protein.

ABSTRACT: Type II polyketide synthases are biosynthetic enzymatic pathways responsible for the production of complex aromatic natural products with important biological activities. In these systems, biosynthetic intermediates are covalently bound to a small acyl carrier protein that associates with the synthase enzymes and delivers the bound intermediate to each active site. In the closely related fatty acid synthases of bacteria and plants, the acyl carrier protein acts to sequester and protect attached intermediates within its helices. Here we investigate the type II polyketide synthase acyl carrier protein from the actinorhodin biosynthetic pathway and demonstrate its ability to internalize the tricyclic, polar molecule emodic acid. Elucidating the interaction of acyl carrier proteins with bound

SUBMITTER: Haushalter RW 

PROVIDER: S-EPMC3092034 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature