Unknown

Dataset Information

0

Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis.

ABSTRACT: Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCR? chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to published TCR from CNS-infiltrating T-cells in MS lesions. These studies are the first description of TCR usage of CNS-specific CD8+ T-cells and provide insights into their potential regulatory role in disease.

SUBMITTER: Biegler BW 

PROVIDER: S-EPMC3092854 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Clonal composition of neuroantigen-specific CD8+ and CD4+ T-cells in multiple sclerosis.

Biegler Brian W BW   Yan Shirley X SX   Ortega Sterling B SB   Tennakoon Deepani K DK   Racke Michael K MK   Karandikar Nitin J NJ  

Journal of neuroimmunology 20110311 1-2

Patients with multiple sclerosis (MS) show a high prevalence of myelin-reactive CD8+ and CD4+ T-cell responses, which are the putative effectors/modulators of CNS neuropathology. Utilizing a novel combination of short-term culture, CFSE-based sorting and anchored PCR, we evaluated clonal compositions of neuroantigen-targeting T-cells from RRMS patients and controls. CDR3 region analysis of TCRβ chains revealed biased use of specific TCRBV-bearing CD4+ clones. CD8+ clones showed homology to publi  ...[more]

Similar Datasets

Unknown Neuroantigen-specific CD8+ regulatory T-cell function is deficient during acute exacerbation of multiple sclerosis.
| S-EPMC3046327 | biostudies-literature
Unknown Neuroantigen-specific autoregulatory CD8+ T cells inhibit autoimmune demyelination through modulation of dendritic cell function.
| S-EPMC4140828 | biostudies-literature
Unknown Increased DNA methylation of SLFN12 in CD4+ and CD8+ T cells from multiple sclerosis patients.
| S-EPMC6209300 | biostudies-literature
Unknown Multiple sclerosis: brain-infiltrating CD8+ T cells persist as clonal expansions in the cerebrospinal fluid and blood.
| S-EPMC356967 | biostudies-literature
Transcriptomics Cell-Type-Specific Transcriptome of CD4+, CD8+ T cells and CD14+ monocytes in multiple sclerosis
2020-12-31 | GSE137143 | GEO
Unknown Suppression of autoimmune demyelinating disease by preferential stimulation of CNS-specific CD8 T cells using Listeria-encoded neuroantigen.
| S-EPMC5431563 | biostudies-literature
Unknown Anti-CD20 therapy depletes activated myelin-specific CD8<sup>+</sup> T cells in multiple sclerosis.
| S-EPMC6926057 | biostudies-literature
Unknown Clonal structure of rapid-onset MDV-driven CD4+ lymphomas and responding CD8+ T cells.
| S-EPMC3088711 | biostudies-other
Unknown Tumor-specific CD4+ T cells maintain effector and memory tumor-specific CD8+ T cells.
| S-EPMC4283993 | biostudies-literature
Unknown Effect of natalizumab on circulating CD4+ T-cells in multiple sclerosis.
| S-EPMC3511477 | biostudies-other