Hyperbaric oxygenation promotes regeneration of biliary cells and improves cholestasis in rats.

ABSTRACT:

Aim

To investigate the effects of hyperbaric oxygenation (HBO) on regeneration of the biliary ductal system and postoperative cholestasis in hepatectomized rats.

Methods

HBO was performed in Wistar rats daily starting 12 h after a 70% partial hepatectomy. Regenerated liver weight, serum parameters and the proliferating cell nuclear antigen labeling index of hepatocytes and biliary ductal cells were measured. Hepatocyte growth factor (HGF), c-Met and transforming growth factor (TGF) β-1 mRNA expression levels were analyzed by quantitative reverse transcription polymerase chain reaction.

Results

HBO improved the postoperative serum levels of total bile acid but not transaminase levels. HBO promoted hepatocyte and biliary ductal cell proliferation. The hematoxylin and eo

SUBMITTER: Idetsu A 

PROVIDER: S-EPMC3092876 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature