The effect of Ku on telomere replication time is mediated by telomere length but is independent of histone tail acetylation.

ABSTRACT: DNA replication in Saccharomyces cerevisiae proceeds according to a temporal program. We have investigated the role of the telomere-binding Ku complex in specifying late replication of telomere-proximal sequences. Genome-wide analysis shows that regions extending up to 80 kb from telomeres replicate abnormally early in a yku70 mutant. We find that Ku does not appear to regulate replication time by binding replication origins directly, nor is its effect on telomere replication timing mediated by histone tail acetylation. We show that Ku instead regulates replication timing through its effect on telomere length, because deletion of the telomerase regulator Pif1 largely reverses the short telomere defect of a yku70 mutant and simultaneously rescues its replication timing defect. Consistent wi

SUBMITTER: Lian HY 

PROVIDER: S-EPMC3093326 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature