Construction of a novel expression cassette for increasing transgene expression in vivo in endothelial cells of large blood vessels.

ABSTRACT: The success of gene therapy hinges on achievement of adequate transgene expression. To ensure high transgene expression, many gene-therapy vectors include highly active virus-derived transcriptional elements. Other vectors include tissue-specific eukaryotic transcriptional elements, intended to limit transgene expression to specific cell types, avoid toxicity and prevent immune responses. Unfortunately, tissue specificity is often accompanied by lower transgene expression. Here, we use eukaryotic (murine) transcriptional elements and a virus-derived posttranscriptional element to build cassettes designed to express a potentially therapeutic gene (interleukin (IL)-10) in large-vessel endothelial cells (ECs) at levels as high as obtained with the cytomegalovirus (CMV) immediate early promote

SUBMITTER: Dronadula N 

PROVIDER: S-EPMC3093449 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature