Trafficking kinesin protein (TRAK)-mediated transport of mitochondria in axons of hippocampal neurons.

ABSTRACT: In neurons, the proper distribution of mitochondria is essential because of a requirement for high energy and calcium buffering during synaptic neurotransmission. The efficient, regulated transport of mitochondria along axons to synapses is therefore crucial for maintaining function. The trafficking kinesin protein (TRAK)/Milton family of proteins comprises kinesin adaptors that have been implicated in the neuronal trafficking of mitochondria via their association with the mitochondrial protein Miro and kinesin motors. In this study, we used gene silencing by targeted shRNAi and dominant negative approaches in conjunction with live imaging to investigate the contribution of endogenous TRAKs, TRAK1 and TRAK2, to the transport of mitochondria in axons of hippocampal pyramidal neurons. We rep

SUBMITTER: Brickley K 

PROVIDER: S-EPMC3093881 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature