Recipes and mechanisms of cellular reprogramming: a case study on budding yeast Saccharomyces cerevisiae.

ABSTRACT:

Background

Generation of induced pluripotent stem cells (iPSCs) and converting one cell type to another (transdifferentiation) by manipulating the expression of a small number of genes highlight the progress of cellular reprogramming, which holds great promise for regenerative medicine. A key challenge is to find the recipes of perturbing genes to achieve successful reprogramming such that the reprogrammed cells function in the same way as the natural cells.

Results

We present here a systems biology approach that allows systematic search for effective reprogramming recipes and monitoring the reprogramming progress to uncover the underlying mechanisms. Using budding yeast as a model system, we have curated a genetic network regulating cell cycle and sporulation. Phenotypic co

SUBMITTER: Ding S 

PROVIDER: S-EPMC3094211 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature