Characterization and genome sequencing of two Propionibacterium acnes phages displaying pseudolysogeny.

ABSTRACT:

Background

Propionibacterium acnes is a Gram positive rod inhabiting the human skin that also infects orthopaedic implants and is associated with acne vulgaris. Previously, one lytic bacteriophage, PA6, from P. acnes has been sequenced and partially characterized. We recently isolated several inducible phages from P. acnes classified as Siphoviruses based on morphology and partial genome sequencing.

Results

In this study we sequenced the inducible P. acnes phages PAD20 and PAS50, isolated from deep infection and from skin, respectively. The genomes of PAD20 and PAS50 are 29,074 and 29,017 bp, respectively, compared with the 29,739 bp of PA6. The phage genomes have 87.3-88.7% nucleotide sequence identity. The genes are divided into clusters with different levels of similarity

SUBMITTER: Lood R 

PROVIDER: S-EPMC3094311 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature