Added value of deep sequencing relative to population sequencing in heavily pre-treated HIV-1-infected subjects.

ABSTRACT:

Objective

To explore the potential of deep HIV-1 sequencing for adding clinically relevant information relative to viral population sequencing in heavily pre-treated HIV-1-infected subjects.

Methods

In a proof-of-concept study, deep sequencing was compared to population sequencing in HIV-1-infected individuals with previous triple-class virological failure who also developed virologic failure to deep salvage therapy including, at least, darunavir, tipranavir, etravirine or raltegravir. Viral susceptibility was inferred before salvage therapy initiation and at virological failure using deep and population sequencing genotypes interpreted with the HIVdb, Rega and ANRS algorithms. The threshold level for mutant detection with deep sequencing was 1%.

Results

7 subjects wi

SUBMITTER: Codoner FM 

PROVIDER: S-EPMC3094345 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature