RMDAP: a versatile, ready-to-use toolbox for multigene genetic transformation.

ABSTRACT:

Background

The use of transgenes to improve complex traits in crops has challenged current genetic transformation technology for multigene transfer. Therefore, a multigene transformation strategy for use in plant molecular biology and plant genetic breeding is thus needed.

Methodology/principal findings

Here we describe a versatile, ready-to-use multigene genetic transformation method, named the Recombination-assisted Multifunctional DNA Assembly Platform (RMDAP), which combines many of the useful features of existing plant transformation systems. This platform incorporates three widely-used recombination systems, namely, Gateway technology, in vivo Cre/loxP and recombineering into a highly efficient and reliable approach for gene assembly. RMDAP proposes a strategy for gene

SUBMITTER: Ma L 

PROVIDER: S-EPMC3094388 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature