The role of actin isoforms in somatic embryogenesis in Norway spruce.

ABSTRACT:

Background

Somatic embryogenesis in spruce is a process of high importance for biotechnology, yet it comprises of orchestrated series of events whose cellular and molecular details are not well understood. In this study, we examined the role of actin cytoskeleton during somatic embryogenesis in Norway spruce line AFO 541 by means of anti-actin drugs.

Results

Application of low doses (50-100 nM) of latrunculin B (Lat B) during the maturation of somatic embryos predominantly killed suspensor cells while leaving the cells in meristematic centres alive, indicating differential sensitivity of actin in the two cell types. The treatment resulted in faster development of more advanced embryos into mature somatic embryos and elimination of insufficiently developed ones. In searching

SUBMITTER: Schwarzerova K 

PROVIDER: S-EPMC3095357 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature