Identification of methylated genes associated with aggressive clinicopathological features in mantle cell lymphoma.

ABSTRACT:

Background

Mantle cell lymphoma (MCL) is genetically characterized by the t(11;14)(q13;q32) translocation and a high number of secondary chromosomal alterations. The contribution of DNA methylation to MCL lymphomagenesis is not well known. We sought to identify epigenetically silenced genes in these tumours that might have clinical relevance.

Methodology/principal findings

To identify potential methylated genes in MCL we initially investigated seven MCL cell lines treated with epigenetic drugs and gene expression microarray profiling. The methylation status of selected candidate genes was validated by a quantitative assay and subsequently analyzed in a series of primary MCL (n = 38). After pharmacological reversion we identified 252 potentially methylated genes. The methylat

SUBMITTER: Enjuanes A 

PROVIDER: S-EPMC3095614 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature