Replication-dependent histone gene expression is related to Cajal body (CB) association but does not require sustained CB contact.

ABSTRACT: Interactions between Cajal bodies (CBs) and replication-dependent histone loci occur more frequently than for other mRNA-encoding genes, but such interactions are not seen with all alleles at a given time. Because CBs contain factors required for transcriptional regulation and 3' end processing of nonpolyadenylated replication-dependent histone transcripts, we investigated whether interaction with CBs is related to metabolism of these transcripts, known to vary during the cell cycle. Our experiments revealed that a locus containing a cell cycle-independent, replacement histone gene that produces polyadenylated transcripts does not preferentially associate with CBs. Furthermore, modest but significant changes in association levels of CBs with replication-dependent histone loci mimic their c

SUBMITTER: Shopland LS 

PROVIDER: S-EPMC30964 | biostudies-literature | 2001 Mar

REPOSITORIES: biostudies-literature