A new pathway of glucocorticoid action for asthma treatment through the regulation of PTEN expression.

ABSTRACT:

Background

"Phosphatase and tensin homolog deleted on chromosome 10" (PTEN) is mostly considered to be a cancer-related gene, and has been suggested to be a new pathway of pathogenesis of asthma. The purpose of this study was to investigate the effects of the glucocorticoid, dexamethasone, on PTEN regulation.

Methods

OVA-challenged mice were used as an asthma model to investigate the effect of dexamethasone on PTEN regulation. Immunohistochemistry was used to detect expression levels of PTEN protein in lung tissues. The human A549 cell line was used to explore the possible mechanism of action of dexamethasone on human PTEN regulation in vitro. A luciferase reporter construct under the control of PTEN promoter was used to confirm transcriptional regulation in response to dexa

SUBMITTER: Ni Z 

PROVIDER: S-EPMC3096598 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature