Synaptically-competent neurons derived from canine embryonic stem cells by lineage selection with EGF and Noggin.

ABSTRACT: Pluripotent stem cell lines have been generated in several domestic animal species; however, these lines traditionally show poor self-renewal and differentiation. Using canine embryonic stem cell (cESC) lines previously shown to have sufficient self-renewal capacity and potency, we generated and compared canine neural stem cell (cNSC) lines derived by lineage selection with epidermal growth factor (EGF) or Noggin along the neural default differentiation pathway, or by directed differentiation with retinoic acid (RA)-induced floating sphere assay. Lineage selection produced large populations of SOX2+ neural stem/progenitor cell populations and neuronal derivatives while directed differentiation produced few and improper neuronal derivatives. Primary canine neural lines were generated from f

SUBMITTER: Wilcox JT 

PROVIDER: S-EPMC3096636 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature