Urinary glycoprotein biomarker discovery for bladder cancer detection using LC/MS-MS and label-free quantification.

ABSTRACT:

Background

Cancers of the urinary bladder are the fifth most commonly diagnosed malignancy in the United States. Early clinical diagnosis of bladder cancer remains a major challenge, and the development of noninvasive methods for detection and surveillance is desirable for both patients and health care providers.

Approach

To identify urinary proteins with potential clinical utility, we enriched and profiled the glycoprotein component of urine samples by using a dual-lectin affinity chromatography and liquid chromatography/tandem mass spectrometry platform.

Results

From a primary sample set obtained from 54 cancer patients and 46 controls, a total of 265 distinct glycoproteins were identified with high confidence, and changes in glycoprotein abundance between groups we

SUBMITTER: Yang N 

PROVIDER: S-EPMC3096687 | biostudies-literature | 2011 May

REPOSITORIES: biostudies-literature