Molecular detection of multiple viral targets in untreated urban sewage from Greece.

ABSTRACT:

Background

Urban sewage virological analysis may produce important information about the strains that cause clinical and subclinical infections in the population, thus supporting epidemiological studies.

Methods

In the present study, a twenty one-month survey (November 2007 to July 2009) was conducted in order to evaluate the presence of human adenoviruses (hAdV), hepatitis A viruses (HAV), hepatitis E viruses (HEV), Noroviruses (NoV), and human Polyomaviruses (hPyV) in untreated sewage samples collected from the inlet of Patras' municipal biological wastewater treatment plant, located in southwestern Greece. Nucleic acid amplification techniques were applied for viral nucleic acid detection. Positive samples were confirmed by sequencing and comparative phylogenetic analysis

SUBMITTER: Kokkinos PA 

PROVIDER: S-EPMC3096948 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature