Differential interleukin-6/Stat3 signaling as a function of cellular context mediates Ras-induced transformation.

ABSTRACT:

Introduction

Tyrosine phosphorylated signal transducer and activator of transcription 3 (pStat3) is present in numerous cancers and is required for mediating tumorigenesis. Autocrine and paracrine interleukin (IL)-6 signaling is the principal mechanism by which Stat3 is persistently phosphorylated in epithelial tumors including breast, lung, colon and gastric cancer. The Ras oncogene mediates cellular transformation without evidence of pStat3 in cultured cells. Recently, however non-tyrosine phosphorylated Stat3 was shown to have a transcriptional activating function, a role in mitochondrial function and to mediate cell migration.. Here we examined the role of Stat3 in Ras mediated transformation.

Methods

Ha-rasV12 transformed mammary epithelial cells (MCF10A) cells were tra

SUBMITTER: Leslie K 

PROVIDER: S-EPMC3096973 | biostudies-literature | 2010

REPOSITORIES: biostudies-literature