Three-dimensional reconstruction of serial mouse brain sections: solution for flattening high-resolution large-scale mosaics.

ABSTRACT: Recent advances in high-throughput technology facilitate massive data collection and sharing, enabling neuroscientists to explore the brain across a large range of spatial scales. One such form of high-throughput data collection is the construction of large-scale mosaic volumes using light microscopy (Chow et al., 2006; Price et al., 2006). With this technology, researchers can collect and analyze high-resolution digitized volumes of whole brain sections down to 0.2 μm. However, until recently, scientists lacked the tools to easily handle these large high-resolution datasets. Furthermore, artifacts resulting from specimen preparation limited volume reconstruction using this technique to only a single tissue section. In this paper, we carefully describe the steps we used to digitally recons

SUBMITTER: Berlanga ML 

PROVIDER: S-EPMC3096995 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature