Establishment of persistent infection with foot-and-mouth disease virus in BHK-21 cells.

ABSTRACT:

Background

Foot-and-mouth disease virus (FMDV) is able to cause persistent infection in ruminants besides acute infection and disease. Since the mechanisms of viral persistence and the determining factors are still unknown, in vitro systems help explore and reveal mechanisms of persistence in vivo by providing useful models for the study of RNA genome mutations and evolution. Ammonium chloride, a lysosomotropic agent that raises intralysosomal pH, reduces the yield of FMDV during infection of BHK-21 cells.

Results

The persistent infection with FMDV serotype O in BHK-21 cells was selected and established readily after treatment of ammonium chloride that acts primarily on the cells. Intact virions were observed located inside the endosomes. Viral genome RNAs and specific prote

SUBMITTER: Huang X 

PROVIDER: S-EPMC3097150 | biostudies-literature | 2011 Apr

REPOSITORIES: biostudies-literature